Modelling Mesenchymal Stem Cell Expansion and Chondrogenic Differentiation
نویسندگان
چکیده
Introduction In vitro expansion and differentiation of mesenchymal stem cells (MSCs) rely on specific environmental conditions. One crucial factor is the oxygen tension. Low oxygen tension improves MSC expansion in vitro. Moreover, it accelerates the induction of a chondrogenic phenotype in subsequent 3D differentiation cultures. However, clones of MSCs from the same donor often differ in their in vitro properties. This kind of heterogeneity has been suggested to originate from an individual decline in MSC function called ‘stem cell ageing’. For therapeutic applications of MSCs in cartilage repair understanding the interdependency of environmental regulation and stem cell aging is crucial.
منابع مشابه
Effects of BIO on proliferation and chondrogenic differentiation of mouse marrow-derived mesenchymal stem cells
In vitro expansion of mesenchymal stem cell (MSCs) into large number is necessary for their application in cell-based treatment of articular cartilage defects. On the other hand, some studies have indicated that BIO (6-Bromoindirubin-3-Oxime) possesses mitogenic effects on cell culture. The objective of the present study was to examine the effect of BIO on in vitro expansion and chondrogenic di...
متن کاملEx vivo Expansion and Differentiation of Mesenchymal Stem Cells from Goat Bone Marrow
Objective(s) Mesenchymal stem cells (MSCs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. The present study tried to isolate and characterize MSCs from goat bone marrow. Materials and Methods Fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. Passaged-3 cells were then different...
متن کاملEquine marrow-derived mesenchymal stem cells: isolation, differentiation and culture optimization
Most studies regarding the marrow-derived equine mesenchymal stem cells (MSCs) have mainly focusedon the cell transplantation without considering the capacity of differentiation and in vitro requirements of thecells. These concerns were investigated in the present study. Equine MSCs were isolated from the sternalmarrow aspirates and expanded through two successive subcultures. Passage-2 equine ...
متن کاملStudy of Chondrogenic Effects of Chondrocytes Cocultured With Murine Bone Marrow-Derived Mesenchymal Stem Cells
Purpose: Co-culture systems of marrow derived mesenchymal stem cells (mMSCs) with mature chondrocytes have theoretically been considered as a putative way of MSCs chondrogenic differentiation. MSCs differentiated in this system could be used for transplantation purpose without of any need to their purification since the cells with which MSCs are co cultured are native cartilage cells. Despite o...
متن کاملتمایز سلول های بنیادی مزانشیمی مشتق از بافت چربی انسانی به سلول های غضروفی با استفاده از داربست هیدروژل فیبرینی
Background and purpose: Mesenchymal stem cells (MSCs) are the most widely used cell sources for cartilage tissue engineering. In the present study, human stem cells were used as a cell source. Scaffolds play an important role in tissue engineering, therefore, this study aimed at evaluating the ability of fibrin scaffolds in chondrogenic differentiation of adipose-derived mesenchymal stem cells ...
متن کامل